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KMID : 0363220070450111154
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Korean Journal of Dermatology
2007 Volume.45 No. 11 p.1154 ~ p.1160
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Antifungal Susceptibility Testing of Candida Species Using ATB FUNGUS 2
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Lee Yong-Hwan
Ha Gyoung-Yim
Suh Moo-Kyu
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Abstract
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Background: Because of recently increasing reports of resistance of Candida (C.) species to antifungal agents, it is necessary to perform antifungal susceptibility testing. ATB FUNGUS 2 (bioMerieux, France) is an easy-to-perform in-vitro antifungal susceptibility test.
Objectives: The purpose of the study was to investigate the minimal inhibitory concentration (MIC) of antifungal agents against Candida species isolated from skin lesion using ATB FUNGUS 2.
Methods: Eighty two clinical isolates of Candida species (including 56 C. albicans, 14 C. parapsilosis, 7 C. guilliermondii, 5 C. glabrata) were testeding ATB FUNGUS 2. MICs against itraconazole (ICZ), fluconazole (FCZ), flucytosine (FC) and amphotericin B (AMP) were read after 24 hours incubation.
Results: MIC of ICZ was <0.125microgram/ml on 73 (89.0%) clinical isolates of Candida species except > or =2.0microgram/ml on four strains (3 C. albicans, 1 C. glabrata) and 0.25~0.5microgram/ml on five strains (1 C. parapsilosis, 3 C. guilliermondii, 1 C. glabrata). MIC of FCZ was <8microgram/ml on 78 (95.1%) Candida species except >128microgram/ml on 3 C. albicans and 16microgram/ml on 1 C. parapsilosis. MIC of FC was <0.5microgram/ml on 76 (92.7%) Candida species except >64microgram/ml on 6 C. albicans. MIC of AMP was all (100.0%) <0.5microgram/ml.
Conclusion: ATB FUNGUS 2 represented a simple and valuable method for antifungal susceptibility testing of Candida species.
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KEYWORD
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ATB FUNGUS 2, Candida species, Superficial candidiasis
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